Biological Characterization of an Italian Isolate of Barley Yellow Dwarf Luteovirus from Barley |
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Authors: | L Guglielmone P Caciagli |
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Institution: | Istituto di Fitovirologia Applicata, CNR, Strada delle cacce 73, 10135 Torino, Italy |
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Abstract: | An isolate of BYDV (BYDV-OC), from barley in Northwest Italy with typical symptoms of yellowing and dwarfing, was transmitted by Rhopalosiphum padi, Sitobion fragariae. S. avenae, Metopolophium festucae, R. maidis and M. dirhodum , but not by Myzus persicae or Schizaphis graminum . It reacted in DAS-ELISA with monoclonal and polyclonal antisera to PAV, but not with antibodies to MAV, RPV and RMV. A polyclonal antiserum prepared to BYDV-OC did not react with MAV-like, RPV-like, or RMV-like isolates of BYDV in ELISA or in Western blots. The concentration of BYDVOC in Avena byzantina plants decreased from weeks 1 to 10 after inoculation, but the total virus content per plant increased up to weeks 7 to 8, following the increase of plant weight. |
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