Esculentoside H inhibits colon cancer cell migration and growth through suppression of MMP-9 gene expression via NF-kB signaling pathway |
| |
Authors: | Sun-Hyung Ha Kyung-Min Kwon Jun-Young Park Fukushi Abekura Young-Choon Lee Tae-Wook Chung Ki-Tae Ha Hyeun Wook Chang Seung-Hak Cho Jong-Suk Kim Cheorl-Ho Kim |
| |
Institution: | 1. Molecular and Cellular Glycobiology Unit, Department of Biological Sciences, SungKyunKwan University, Chunchun-Dong, Jangan-Gu, Suwon, Kyunggi-Do, Korea;2. Department of Biological Sciences, Faculty of Medicinal Biotechnology, Dong-A University, Busan, Republic of Korea;3. Division of Applied Medicine, Healthy Aging Korean Medical Research Center, School of Korean Medicine, Pusan National University, Yangsan, Gyeongsangnam-do, Republic of Korea;4. Department of Pharmacy, Yeungnam University, Daehak-Ro, Gyeongsan, Gyeongbuk, Republic of Korea;5. Division of Bacterial Disease Research, Center for Infectious Disease Research, Korea National Institute of Health, Cheongju, Korea;6. Department of Biochemistry, Institute for Medical Sciences, Chonbuk National University Medical School, Baekje-daero, Deokjin-gu, Jeonju, Korea |
| |
Abstract: | A water-soluble saponin, Esculentoside H (EsH), 3-O-(O-β-d -glucopyranosyl-(1→4)-β-d -xylopyranosyl)-28-β-d -glucopyranosylphytolaccagenin has been isolated and purified from the root extract of perennial plant Phytolacca esculenta. EsH is known to be an anticancer compound, having a capacity for TNF-α release. However, the effects of EsH on migration and growth in tumor cells have not yet been reported. In the current study, the suppressive effects of EsH on phorbol 12-myristate 13-acetate (PMA)-induced cell migration were examined in murine colon cancer CT26 cells and human colon cancer HCT116 cells. Interestingly, the transwell assay and wound healing show that EsH suppresses the PMA-induced migration and growth potential of HCT116 and CT26 colon cancer cells, respectively. EsH dose-dependently suppressed matrix metalloproteinases-9 (MMP-9) expression that was upregulated upon PMA treatment in messenger RNA levels and protein secretion. Since the expression of MMP-9 is correlated with nuclear factor-κB (NF-κB) signaling, it has been examined whether EsH inhibits PMA-induced IκB phosphorylation that leads to the suppression of NK-κB nuclear translocation. EsH repressed the phosphorylation level of JNK, but not extracellular signal-regulated kinase and p38 signaling when the cells were treated with PMA. Overall, these results demonstrated that EsH could suppress cancer migration through blockage of the JNK1/2 and NF-κB signaling-mediated MMP-9 expression. |
| |
Keywords: | cancer cancer growth colon cancer migration mitogen-activated protein kinase-9 |
|
|