Efficiently accumulating germ-like stem cells from mouse postnatal ovary by in situ tissue culture |
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Authors: | Meizi Zhang Li Liu Xiaomin Cao Ye Liu Jianyong Di Xiuying Huang Fangzhen Sun Weihong Huang Fengqin Xu |
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Institution: | 1. Reproductive Medicine Center, Tianjin First Central Hospital, Tianjin, China;2. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China |
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Abstract: | Although recent studies have revealed that germline stem cells (GSCs) exist in the mouse postnatal ovary, how to efficiently obtain GSCs for regenerating neo-oogenesis is still a technical challenge. Here, we report that using in situ tissue culture we can efficiently accumulate large amounts of proliferating germ-like cells from mouse postnatal ovaries. Usually, more than 10,000 germ-like cells can be derived from one ovary by this method, and over 20% of these cells can grow into germ-like cells with self-renewal, which thus can serve as a good cell pool to isolate GSCs by other cell assorting methods such as FACS. This method is simple and time-saving, which should be useful for in future studies on mouse GSCs. |
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Keywords: | germline stem cells in situ tissue culture postnatal mouse ovary proliferation |
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