Changes in Activities of Glucose Metabolising Enzymes in Germ Cells during Spermatogenesis in Rats |
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| Authors: | MASAHISA NAKAMURA AKIKO FUJIWARA IKUO YASUMASU SHOICHI OKINAGA KIYOSHI ARAI |
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| Institution: | Department of Obstetrics and Gynecology, School of Medicine, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo 173, Japan;Department of Biology, School of Education, Waseda University, 1-6-1 Nishi-Waseda, Shinjuku-ku, Tokyo 160, Japan |
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| Abstract: | The rate of 14CO2, liberation from 14C-1]glucose was identical to that from 14C-6]glucose in spermatids, but more than the latter in spermatogonia. Rotenone (1 μM) completely inhibited 14CO2 release from 14C-1]glucose in spermatids, but decreased it only 30% in spermatogonia. The activity of glucose-6-phosphate dehydrogenase, but not 6-phosphogluconate dehydrogenase, was markedly lower in spermatocytes and spermatids than in spermatogonia. The activities of the glycolytic enzymes, glucosephosphate isomerase, fructose diphosphatase, glyceraldehyde-3-phosphate dehydrogenase and enolase, differed only slightly in spermatids and spermatogonia. It is concluded that the low glucose-6-phosphate dehydrogenase activity may contribute to the low activity of the pentose cycle in spermatocytes and spermatids. |
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