Multiplexed genotyping of ABC transporter polymorphisms with the Bioplex suspension array |
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| Authors: | Seok Hwee Koo Tan Ching Ong Kok Ting Chong Caroline Guat Lay Lee Fook Tim Chew Edmund Jon Deoon Lee |
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| Institution: | (1) Department of Biological Sciences, National University of Singapore, Singapore;(2) Department of Biochemistry, National University of Singapore, Singapore;(3) Department of Pharmacology, National University of Singapore. Block MD11, Level 5, #05-09, Clinical Research Centre, 10 Medical Drive, 117597 Singapore |
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| Abstract: | We have developed and validated a consolidated bead-based genotyping platform, the Bioplex suspension array for simultaneous
detection of multiple single nucleotide polymorphisms (SNPs) of the ATP-binding cassette transporters. Genetic polymorphisms
have been known to influence therapeutic response and risk of disease pathologies. Genetic screening for therapeutic and diagnostic
applications thus holds great promise in clinical management. The allele-specific primer extension (ASPE) reaction was used
to assay 22 multiplexed SNPs for eight subjects. Comparison of the microsphere-based ASPE assay results to sequencing results
showed complete concordance in genotype assignments. The Bioplex suspension array thus proves to be a reliable, cost-effective
and high-throughput technological platform for genotyping. It can be easily adapted to customized SNP panels for specific
applications involving large-scale mutation screening of clinically relevant markers. |
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| Keywords: | Indexing terms" target="_blank">Indexing terms Genotype Microspheres Polymorphism Genetic |
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