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The chlorophyllases AtCLH1 and AtCLH2 are not essential for senescence-related chlorophyll breakdown in Arabidopsis thaliana |
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| Authors: | Schenk Nicole Schelbert Silvia Kanwischer Marion Goldschmidt Eliezer E Dörmann Peter Hörtensteiner Stefan |
| Institution: | a Institute of Plant Sciences, University of Bern, Altenbergrain 21, CH-3013 Bern, Switzerland b Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, CH-8008 Zurich, Switzerland c Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, D-14476 Golm, Germany d The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot 76100, Israel |
| Abstract: | One important reaction of chlorophyll (chl) breakdown during plant senescence is the removal of the lipophilic phytol moiety by chlorophyllase. AtCLH1 and AtCLH2 were considered to be required for this reaction in Arabidopsis thaliana. Here we present evidence against this assumption. Using green fluorescent protein fusions, neither AtCLH isoform localizes to chloroplasts, the predicted site of chlorophyll breakdown. Furthermore, clh1 and clh2 single and double knockout lines are still able to degrade chlorophyll during senescence. From our data we conclude that AtCLHs are not required for senescence-related chlorophyll breakdown in vivo and propose that genuine chlorophyllase has not yet been molecularly identified. |
| Keywords: | chl chlorophyll Chlide chlorophyllide FCC fluorescent chlorophyll catabolite GFP green fluorescent protein NCC non-fluorescent chlorophyll catabolite PAO pheophorbide a oxygenase RCCR red chlorophyll catabolite reductase |
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