L protease from foot and mouth disease virus confers eIF2-independent translation for mRNAs bearing picornavirus IRES |
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Authors: | Pablo Moral-López Enrique Alvarez Natalia Redondo Tim Skern Luis Carrasco |
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Institution: | 1. Centro de Biología Molecular Severo Ochoa, (CSIC-UAM), C/Nicolás Cabrera, 1, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain;2. Max F. Perutz Laboratories, Medical University of Vienna, Vienna, Austria |
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Abstract: | The leader protease (Lpro) from foot-and-mouth disease virus (FMDV) has the ability to cleave eIF4G, leading to a blockade of cellular protein synthesis. In contrast to previous reports, our present findings demonstrate that FMDV Lpro is able to increase translation driven by FMDV IRES. Additionally, inactivation of eIF2 subsequent to phosphorylation induced by arsenite or thapsigargin in BHK cells blocks protein synthesis directed by FMDV IRES, whereas in the presence of Lpro, significant translation is found under these conditions. This phenomenon was also observed in cell-free systems after induction of eIF2 phosphorylation by addition of poly(I:C). |
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Keywords: | Picornavirus IRES eIF2 Regulation of translation FMDV L protease Poliovirus 2A |
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