Evidence that the C-terminus of OprM is involved in the assembly of the VceAB-OprM efflux pump |
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Authors: | Jiangping Bai Lakysha Mosley |
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Institution: | a Department of Microbiology and Immunology, Texas Tech University Health Science Center, TX 79430, USA b Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou 730070, China |
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Abstract: | Although the architecture of tripartite multiple drug resistance (MDR) efflux pumps of Gram-negative bacteria has been well characterized, the means by which the components recognize each other and assemble into a functional pump remains obscure. In this study we present evidence that the C-terminal domain of the Pseudomonas aeruginosa OprM and the α-helical hairpin domain of Vibrio cholerae VceA play an important role in the recognition/specificity/recruitment step in the assembly of a functional, VceAB-OprM chimeric efflux pump. To our knowledge, this is the first evidence directly linking the C-terminal domain of an outer membrane efflux protein to its recruitment during the assembly of a tripartite efflux pump. |
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Keywords: | Involvement of C-terminus of OprM in assembly of VceAB-OprM |
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