LOX-1 mediates lysophosphatidylcholine-induced oxidized LDL uptake in smooth muscle cells |
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| Authors: | Aoyama T Chen M Fujiwara H Masaki T Sawamura T |
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| Institution: | Second Department of Internal Medicine, Gifu University School of Medicine, Gifu, Japan. |
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| Abstract: | To assess the role of 14-3-3 proteins in the magnesium-dependent inhibition of nitrate reductase (NR) we tested the effect of magnesium on NR binding to 14-3-3s by coimmunoprecipitation and gel filtration. The stability of the 14-3-3 complex of NR was, unlike its activity, unaffected by magnesium. We therefore conclude that binding to 14-3-3s per se does not inhibit NR. Magnesium inhibited 14-3-3-bound NR much more strongly than 14-3-3-free NR. 14-3-3s possibly reinforce NR inhibition by magnesium. |
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| Keywords: | 14-3-3 Protein Nitrate reductase Protein phosphorylation Protein–protein interaction Enzyme inhibition |
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