Induction of growth arrest by miR-542-3p that targets survivin |
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Authors: | Sena Yoon Suman Lee Jaeseung Yoon |
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Institution: | a Graduate School of Biotechnology, Kyung Hee University, Yongin, Republic of Korea b Cell and Gene Therapy Research Institute, School of Medicine, CHA University, Seongnam-si, Republic of Korea |
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Abstract: | Survivin is a protein which functions as a mitotic regulator as well as apoptosis inhibitor. In this study, we show that introduction of synthetic miR-542-3p mimetic reduced both mRNA and protein levels of survivin. In A549 cells, luciferase reporter assay revealed that miR-542-3p targeted predicted binding sites in the 3′-untranslated region (3′-UTR) of survivin. We also demonstrate that ectopic expression of miR-542-3p inhibited cell proliferation by inducing Gap 1 (G1) and Gap 2/Mitosis (G2/M) cell cycle arrest. Collectively, these results suggest that survivin is a direct target of miR-542-3p and growth inhibition by miR-542-3p may have a potential utility as an anti-cancer therapy. |
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Keywords: | miRNA microRNA G1 Gap 1 G2/M Gap 2/Mitosis 3&prime -UTR 3&prime -untranslated region CDK cyclin-dependent kinase E2F3 E2 promoter binding factor 3 IAP inhibitor of apoptosis CPC chromosomal passenger complex rRNA ribosomal RNA |
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