首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Anti-HIV-1 property of trichosanthin correlates with its ribosome inactivating activity
Authors:Wang Jian Hua  Nie Hui Ling  Tam Siu Cheung  Huang Hai  Zheng Yong Tang
Institution:Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, PR China.
Abstract:Trichosanthin (TCS) is a type I ribosome inactivating (RI) protein possessing anti-tumor and antiviral activity, including human immunodeficiency virus (HIV). The mechanism of these actions is not entirely clear, but is generally attributed to its RI property. In order to study the relationship between the anti-HIV-1 activity of TCS and its RI activity, three TCS mutants with different RI activities were constructed by using site-directed mutagenesis. The anti-HIV-1 activities of the three mutants were tested in vitro. Results showed that two TCS mutants, namely TCS(M(120-123)), TCS(E160A/E189A), with the greatest decrease in RI activity, lost almost all of the anti-HIV activity and cytopathic effect. Another mutant TCS(R122G), which exhibited a 160-fold decrease in RI activity, retained some anti-HIV activity. The results from this study suggested that RI activity of TCS may have significant contribution to its anti-HIV-1 property.
Keywords:Trichosanthin  Ribosome inactivating protein  human immunodeficiency virus type 1  Antiviral activity
本文献已被 ScienceDirect PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号