Human initiation factor eIF3 subunit b interacts with HCV IRES RNA through its N-terminal RNA recognition motif |
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Authors: | Julien Pérard Jan Medenbach Jérôme Boisbouvier Florence Baudin |
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Institution: | a Unit of Virus Host-Cell Interactions, UJF-EMBL-CNRS (UMR 5233), 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France b Institut de Biologie Structurale (IBS) J.P. Ebel, UJF-CEA-CNRS (UMR 5075), 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France c European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany |
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Abstract: | Many viral mRNAs contain a 5′-UTR RNA element called internal ribosome-entry site (IRES), which bypasses the requirement of some canonical initiation factors allowing cap-independent translation. The IRES of hepatitis-C virus drives translation by directly recruiting 40S ribosomal subunits and binds to eIF3 which plays a critical role in both cap-dependent and cap-independent translation. However, the molecular basis for eIF3 activity in either case remains enigmatic. Here we report that subunit b of the eIF3 complex directly binds to HCV IRES domain III via its N-terminal-RRM. Because eIF3b was previously shown to be involved in eIF3j binding, biological implications are discussed. |
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Keywords: | eIF3b HCV IRES RNA NMR spectroscopy RNA-protein interaction Translation initiation |
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