Arabidopsis thaliana expresses a second functional flavonol synthase |
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Authors: | Anja Preuß Bernd Weisshaar Ulrich Matern |
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Institution: | a Philipps-Universität Marburg, Institut für Pharmazeutische Biologie, Deutschhausstrasse 17A, D-35037 Marburg/Lahn, Germany b Bielefeld University, Faculty of Biology, Chair of Genome Research, Universitaetsstrasse27, D-33615 Bielefeld, Germany c Philipps-Universität Marburg, Institut für Pharmazeutische Chemie, Marbacher Weg 6, D-35037 Marburg/Lahn, Germany |
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Abstract: | Arabidopsis thaliana L. produces flavonoid pigments, i.e. flavonols, anthocyanidins and proanthocyanidins, from dihydroflavonol substrates. A small family of putative flavonol synthase (FLS) genes had been recognized in Arabidopsis, and functional activity was attributed only to FLS1. Nevertheless, other FLS activities must be present, because A. thalianafls1 mutants still accumulate significant amounts of flavonols. The recombinant FLSs and leucoanthocyanidin dioxygenase (LDOX) proteins were therefore examined for their enzyme activities, which led to the identification of FLS3 as a second active FLS. This enzyme is therefore likely responsible for the formation of flavonols in the ldox/fls1-2 double mutant. These double mutant and biochemical data demonstrate for the first time that LDOX is capable of catalyzing the in planta formation of flavonols. |
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Keywords: | FLS flavonol synthase LDOX leucoanthocyanidin dioxygenase 2-ODD 2-oxoglutarate dependent dioxygenases PA proanthocyanidins TLC thin-layer chromatography |
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