Maintenance of luminal NADPH in the endoplasmic reticulum promotes the survival of human neutrophil granulocytes |
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Authors: | Kardon Tamás Senesi Silvia Marcolongo Paola Legeza Balázs Bánhegyi Gábor Mandl József Fulceri Rosella Benedetti Angelo |
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Institution: | Semmelweis Egyetem Orvosi Vegytani, Molekuláris Biológiai és Patobiokémiai Intézete & MTA-SE Patobiokémiai Kutatócsoport, Budapest, Hungary. |
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Abstract: | The present study demonstrates the expression of hexose-6-phosphate dehydrogenase and 11 beta-hydroxysteroid dehydrogenase type 1 in human neutrophils, and the presence and activity of these enzymes in the microsomal fraction of the cells. Their concerted action together with the previously described glucose-6-phosphate transporter is responsible for cortisone-cortisol interconversion detected in human neutrophils. Furthermore, the results suggest that luminal NADPH generation by the cortisol dehydrogenase activity of 11 beta-hydroxysteroid dehydrogenase type 1 prevents neutrophil apoptosis provoked by the inhibition of the glucose-6-phosphate transporter. In conclusion, the maintenance of the luminal NADPH pool is an important antiapoptotic factor in neutrophil granulocytes. |
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Keywords: | Apoptosis 11β-Hydroxysteroid dehydrogenase type 1 Hexose-6-phosphate dehydrogenase Glucose-6-phosphate transporter Glucocorticoid |
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