Glutamate-induced glioma cell proliferation is prevented by functional expression of the glutamate transporter GLT-1 |
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Authors: | Vanhoutte Nicolas Hermans Emmanuel |
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Institution: | Laboratoire de Pharmacologie Expérimentale, Université catholique de Louvain, 54.10, Av. Hippocrate 54, 1200 Brussels, Belgium. |
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Abstract: | A tetracycline-dependent inducible system was used to achieve controlled expression of the glutamate transporter 1 (GLT-1) in C6 glioma cells. Non-induced cells show modest glutamate uptake and, in the presence of L-cystine, these cells tend to release substantial amounts of glutamate. Overnight exposure to doxycycline increased D-3H]-aspartate uptake, reaching similar capacity as observed in cultured astrocytes. Efficient clearance of exogenously applied glutamate was evidenced in these cells, even in the presence of l-cystine. The addition of glutamate (100 microM) to the medium of non-induced cells significantly increased their proliferation rate, an effect that was blocked when the expression of GLT-1 was induced. This suggests that impaired glutamate uptake capacity in glioma cells indirectly contributes to their proliferation. |
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