Characterization of ScORK28, a transmembrane functional protein receptor kinase predominantly expressed in ovaries from the wild potato species Solanum chacoense |
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Authors: | Germain Hugo Houde Josée Gray-Mitsumune Madoka Sawasaki Tatsuya Endo Yaeta Rivoal Jean Matton Daniel P |
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Institution: | Institut de Recherche en Biologie Végétale (IRBV), Département de Sciences Biologiques, Université de Montréal, 4101 Sherbrooke Est, Montréal, QC, Canada H1X 2B2. |
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Abstract: | Solanum chacoense ovule receptor kinase 28 (ScORK28) was found among 30 receptor kinases from an ovule cDNA library enriched for weakly expressed mRNAs. This LRR-RLK displayed high level of tissue specificity at the RNA and protein levels and was predominantly expressed in female reproductive tissues. Protein expression analyses in planta revealed that ScORK28 was N-glycosylated and ScORK28::GFP fusion analyses showed that it was localized at the plasma membrane. Bacterial expression of ScORK28 catalytic domain followed by kinase activity assays revealed that ScORK28 is an active Mg2+-dependent protein kinase and that the juxtamembrane domain is necessary for kinase activity. |
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Keywords: | Receptor-like kinase Ovule Fertilization Solanum chacoense |
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