The detection and characterization of G-proteins in the eyespot of Chlamydomonas reinhardtii |
| |
Authors: | SN Korolkov MN Garnovskaya AS Basov AS Chunaev IL Dumler |
| |
Institution: | Sechenov Institute of Evolutionary Physiology and Biochemistry, USSR Academy of Sciences, Leningrad. |
| |
Abstract: | The presence of G-proteins in the eyespot fraction of Chlamydomonas reinhardtii is shown. This fraction is capable of binding (GTPγ35S], possesses the GTPase activity and interacts with antibodies raised against a highly conserved peptide of most G-proteins' -subunit. Cross-reaction with a 24-kDa protein is detected on immunoblots. Using an antiserum prepared from vertebrate β-subunit peptide, two additional proteins with apparent Mr 21 and 29 kDa could be revealed. The light-dependence of GTPase extraction from eyespot membranes is shown. The results make it possible to suggest the participation of G-proteins in the photosensory transduction chain of Ch. reinhardtii. |
| |
Keywords: | G-protein GTPase Rhodopsin GTPγ[35S] Chlamydomonas reinhardtii |
本文献已被 ScienceDirect 等数据库收录! |