An interaction between human Sec63 and nucleoredoxin may provide the missing link between the SEC63 gene and polycystic liver disease |
| |
Authors: | Müller Linda Funato Yosuke Miki Hiroaki Zimmermann Richard |
| |
Institution: | aMedical Biochemistry and Molecular Biology, Saarland University, 66421 Homburg, Germany;bLaboratory of Intracellular Signaling, Institute for Protein Research, Osaka University, Osaka 565-0871, Japan |
| |
Abstract: | The formation of multiple cysts in one or several organs is a characteristic of several human inherited diseases. Recent research suggests that problems in planar cell polarity may be the common denominator in polycystic diseases. Mutations in at least two genes are linked to autosomal dominant polycystic liver disease (PCLD), PRKCSH and SEC63. A recent study linked PRKCSH to the signaling- and cytoskeletal adaptor-component β-catenin. In a yeast two hybrid screen we identified the cytosolic protein nucleoredoxin (NRX) as an interaction partner of human Sec63. Since NRX is involved in the Wnt signaling pathways, we characterized this interaction. Thus, Sec63 is linked to the Wnt signaling pathways and this interaction may be the reason why mutations in SEC63 can lead to PCLD.Structured summarySec63physically interacts with NRX by two hybrid(View interaction)NRXbinds to Sec63 by peptide array (View Interaction 1, 2)Sec63binds to NRX by pull down(View interaction)Sec63binds to NRX by peptide array (View Interaction 1, 2, 3) |
| |
Keywords: | Abbreviations: ER endoplasmic reticulum GST glutathione S-transferase NRX nucleoredoxin PCLD polycystic liver disease |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|