Tetrameric structure of the nonactivated glucocorticoid receptor in cell extracts and intact cells |
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Authors: | M Rexin W Busch B Segnitz U Gehring |
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Institution: | Institut für Biologische Chemie, Universit?t, Heidelberg, FRG. |
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Abstract: | Mouse lymphoma cells contain a nonactivated glucocorticoid receptor of Mr approximately 330,000 which is heteromeric in nature and is unable to bind to DNA. Following affinity labeling of the steroid-binding subunit and subsequent cross-linking with dimethyl suberimidate at various times either in cell extracts or in intact cells, a series of labeled bands was detected in SDS gels. From the molecular masses of completely and partially cross-linked complexes we conclude that the large nonactivated receptor is a tetramer composed of two 90 kDa subunits, one 50 kDa polypeptide and one steroid-binding subunit. |
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