Identification of 4-chlorobenzoyl-coenzyme A as intermediate in the dehalogenation catalysed by 4-chlorobenzoate dehalogenase from Pseudomonas sp. CBS3 |
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Authors: | Frank Lffler Rudolf Müller |
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Institution: | Frank Löffler,Rudolf Müller |
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Abstract: | The intermediate in the reaction catalysed by 4-chlorobenzoate dehalogenase from Pseudomonas sp. CBS3 was identified as 4-chlorobenzoyl-CoA. One component of 4-chlorobenzoate debalogenase worked as a a 4-chlorobenzoyl-CoA ligase catalysing the formation of 4-chlorobenzoyl-CoA from 4-chlorobenzoate, coenzyme A and ATP. This intermediate was detected spectrophotometrically and by HPLC. 4-chlorobenzoyl-CoA was the substrate for the dehalogenase component, which catalysed the conversion to 4-hydroxybenzoate with concomitant release or coenzyme A. |
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Keywords: | 4-Chlorobenzoate 4-Chlorobenzoate dehalogenase 4-Chlorobenzoyl-CoA: Dehalogenation: Degradation of chlorinated hydrocarbons Pseudomonas sp CBS3 |
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