Sensitive determination of vinorelbine and its metabolites in human serum using liquid chromatography-electrospray mass spectrometry |
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| Authors: | S Ragot F L Sauvage A Rousseau D Genet J L Dupuy N Tubiana-Mathieu P Marquet |
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| Institution: | a Department of Pharmacology and Toxicology, University Hospital, CHU Dupuytren, 2 Avenue Martin-Luther-King, 87042 Limoges cedex, France;b Department of Oncology, 87042 Limoges cedex, France;c Laboratory of Biophysics, Faculty of Pharmacy, 87025 Limoges cedex, France |
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| Abstract: | A liquid chromatography-electrospray mass spectrometry method was developed for the quantitation of vinorelbine (VNB) and two metabolites, vinorelbine N-oxide (VNO) and deacetyl vinorelbine (DAV) in human serum. The limits of quantitation (LOQ) reached 0.5 ng/ml for both VNB and VNO and 1 ng/ml for DAV. The method was proved linear in the range of LOQs up to 1000 ng/ml, and extraction recovery was 80% on average for the three compounds. It was applied to the pharmacokinetic monitoring of vinorelbine and, for the first time, to the detection of VNO in the serum of patients suffering from non-small-cell lung cancer. |
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| Keywords: | Vinorelbine Vinorelbine N-oxide Deacetylvinorelbine LC-MS |
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