Design and applications of a self-aligning liquid junction-electrospray interface for capillary electrophoresis-mass spectrometry |
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Authors: | Timothy Wachs Robin L Sheppard Jack Henion |
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Institution: | aAnalytical Toxicology, Diagnostic Laboratory, Cornell University, 927 Warren Drive, Ithaca, NY 14850, USA;bOn sabbatical from Xerox Corporation, 800 Phillips Road, 139-64A, Webster, NY 14580, USA |
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Abstract: | A simple self-aligning liquid junction-electrospray interface for coupling a capillary electrophoresis (CE) system to an atmospheric pressure ionization (API) mass spectrometer (CE-MS) was developed. In contrast to previous liquid junction interfaces, the self-aligning liquid junction interface simplifies the precise alignment of the CE capillary and the sprayer needle and uses a positive make-up flow. Several capillary CE-MS applications were run using both the self-aligning liquid junction interface and the widely used sheath flow interface for comparison purposes. The electrospray stability of the self-aligning liquid junction interface is consistently better even when non-volatile electrolyte solutions are used. At first, some band broadening was obtained with the self-aligning liquid junction interface. Experiments with different CE buffer systems suggested that this band broadening was caused by the materials used in constructing the interface. By using a more inert material for the sprayer needle, the self-aligning liquid junction exhibits excellent electrophoretic resolution, comparable sensitivity, and higher signal-to-noise ratios when run under the same conditions as the sheath flow interface. |
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Keywords: | Self-aligning liquid junction-electrospray interface Ephedrine Isoproternol Orciprenaline Terbutaline Tulobuterol Salbutamol Clenbuterol Ritodrine Fenoterol |
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