Capillary zone electrophoresis for the determination of atovaquone in serum |
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Authors: | Chi-Chung Chou Murray P Brown Kelly A Merritt |
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Institution: | Department of Large Animal and Clinical Sciences, College of Veterinary Medicine, University of Florida, P.O. Box 100136, Gainesville, FL 32610, USA |
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Abstract: | A rapid and simple capillary zone electrophoresis (CZE) method has been developed for the determination of atovaquone in serum. The drug was extracted from equine serum–chloroform (1:3, v/v) at greater than 80% recovery and assayed in buffer containing 25 mM sodium borate (pH 9.1) and 25% acetonitrile. A 100 μm I.D. fused-silica capillary was used and the detection was by UV-diode array at 254 nm; the migration time was approximately 8 min. Intra- and inter-assay variabilities were less than 7.8% and 5.8%, respectively, and the accuracy of the assay (expressed as % bias) ranged from 4.5 to −5.2%. The working assay range was from 2 to 100 μg/ml. This sensitivity could be increased by concentrating during the extraction procedure. Replacement of acetonitrile with 75 mM surfactant 3-(dimethyldodecylammonio)propanesulfonate gave similar sensitivity and provided an additional option to facilitate the separation of atovaquone on multiple-drug samples. |
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Keywords: | Atovaquone |
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