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Method for detecting the lectin activity of Momordica charantia transferred from micro two-dimensional electrophoretic gel on to nitrocellulose |
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| Authors: | Toshinori Omi Toyomi Kamesaki Eiji Kajii and Shigenori Ikemoto |
| Institution: | Department of Legal Medicine and Human Genetics, Jichi Medical School, Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi-ken 329-04 Japan Department of Internal Medicine, Tottori Prefectural Central Hospital, 730 Ezu, Tottori 680 Japan Department of Legal Medicine and Human Genetics, Jichi Medical School, Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi-ken 329-04 Japan |
| Abstract: | A method was devised for detecting both the molecular mass and the isoelectric point (pI) of the lectin in the seed extract of Momordica charantia on a nitrocellulose membrane. It was associated with the electrophoretic blotting technique that produced replicas of proteins separated on micro two-dimensional polyacrylamide gels. The red blood cell adherence procedure on the blotted membrane exhibited only one red spot with molecular mass 107·103 and pI 5.3, which indicated the lectin activity. Additionally, the lectin appeared to be a glycoprotein with mannose and/or glucose, because it was stained by concanavalin A-peroxidase staining. |
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