Aqueous two-phase systems extraction of alpha-toxin from Clostridium perfringens type A |
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Authors: | Cavalcanti Maria Taciana Holanda Porto Tatiana Souza de Barros Neto Benicio Lima-Filho José Luiz Porto Ana Lúcia Figueiredo Pessoa Adalberto |
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Institution: | Departament of Biochemical and Pharmaceutical Technology, University of S?o Paulo, Av. Prof. Lineu Prestes, 580, B.16. 05508-000, S?o Paulo, SP, Brazil. |
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Abstract: | Two sequential half-fraction designs were applied to studying the alpha-toxin partition produced by Clostridium perfringens type A in aqueous two phase systems (ATPS), as a function of four factors: PEG molar mass and concentration, phosphate concentration and pH. The highest purification factor, yield and partition coefficient results were obtained with PEG 8000 (15%, w/w), phosphate at 20% (w/w) and pH 8.0. This system allows, in a single step, an alpha-toxin purification of 4.6-fold with final activity yield of 230% and partition coefficient of 113.9 in the PEG rich phase. |
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