Application of high-speed counter-current chromatography for preparative separation of cyclic peptides from Vaccaria segetalis |
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Authors: | Wang Xiao Dong Hongjing Liu Yuqin Yang Bin Wang Xia Huang Luqi |
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Institution: | Institute of Chinese Material Medical, China Academy of Chinese Medical Sciences, Beijing, China. |
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Abstract: | Following an initial clean-up step on silica gel, high-speed counter-current chromatography (HSCCC) was used to separate cyclic peptides from an extract of the seeds of Vaccaria segetalis. The two-phase solvent system used for HSCCC separation was composed of petroleum ether-ethyl acetate-methanol-water at an optimized volume ratio of 0.5:3.5:1:5. From 190 mg of crude extract, 38.0 mg of segetalin B and 28.5 mg of segetalin A were obtained with purities of 98.1% and 95.6% as determined by HPLC, respectively. The chemical structures of the target compounds were confirmed by high resolution electrospray ionization time of flight MS (HRESI-TOF-MS) and (1)H NMR analyses. |
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