High-performance liquid chromatographic separation of bile acid pyrenacyl esters with cyclodextrin-containing mobile phase |
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| Authors: | Kazutake Shimada Yoshihiro Komine Kuniko Mitamura |
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| Institution: | Kazutake Shimada*, Yoshihiro Komine,Kuniko Mitamura |
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| Abstract: | The high-performance liquid chromatographic separation of bile acid pyrenacyl esters with cyclodextrin-containing mobile phase is presented. Compared with conventional methods, inclusion chromatography gives much more satisfactory separation of derivatized bile acids in a short time. The application of this method to the separation of glycine-conjugated bile acids in human bile is also described. |
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