A novel approach for the construction of multiple shRNA expression vectors |
| |
Authors: | Gou Deming Weng Tingting Wang Yang Wang Zhixin Zhang Honghao Gao Li Chen Zhongming Wang Pengcheng Liu Lin |
| |
Institution: | Department of Physiological Sciences, Oklahoma State University, Stillwater, OK 74078, USA. |
| |
Abstract: | The application of RNA interference (RNAi) as a research and therapeutic tool depends on its ability to silence genes in a sequence-specific manner. Recent studies have reported that the effective knockdown of genes can be achieved by multiple short hairpin RNAs (shRNAs) in a single vector. Moreover, this approach can depress several genes simultaneously. However, current methods for the construction of multiple shRNA vectors often suffer from vector instability and are time-consuming. Here, we describe a simple, quick and low-cost approach to construct a single vector expressing four shRNA sequences driven by four different promoters. Using this vector, we were able to improve the gene silencing efficiency and make it possible to silence four different genes simultaneously, further expanding the application spectrum of RNAi, both in functional studies and therapeutic strategies. |
| |
Keywords: | RNA interference gene silencing gene therapy short interfering RNA short hairpin RNA |
本文献已被 PubMed 等数据库收录! |
|