| 高效液相色谱法测定辣椒干中辣椒素的含量 |
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| 引用本文： | 高艺,钟世安,周春山,陈龙胜.高效液相色谱法测定辣椒干中辣椒素的含量[J].天然产物研究与开发,2005,17(6):797-799. |
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| 作者姓名： | 高艺 钟世安 周春山 陈龙胜 |
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| 作者单位： | 中南大学化学化工学院,长沙,410083 |
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| 基金项目： | Project supported by the Natural Science Foundation of Hunan Province（No. 04JJ3080） |
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| 摘 要： | 本文建立了简便、快速的分离测定辣椒干中辣椒素含量的高效液相色谱法.HPC的条件Burospher-100 C18柱(I.D.250 mm×4.6 mm,5μm);流动相为甲醇-水(7030,V/V);流速为0.6 mL/min;检测波长为280 nm.在上述条件下,辣椒素在1.01～121.2 mg/L的范围内线性关系良好,线性相关系数γ=0.9994;实验结果表明,该方法的相对标准偏差在1.38%(n=6)以内,平均回收率为99.88%.
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| 关 键 词： | 高效液相色谱 辣椒 辣椒素 定量分析 |
| 收稿时间： | 2005-02-22 |
| 修稿时间： | 2005-05-27 |
Determination of Capsaicin in Chilli ( Capsicum annuum L. ) by HPLC |
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| GAO Yi,ZHONG Shi-an,ZHOU Chun-shan,CHEN Long-sheng.Determination of Capsaicin in Chilli ( Capsicum annuum L. ) by HPLC[J].Natural Product Research and Development,2005,17(6):797-799. |
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| Authors: | GAO Yi ZHONG Shi-an ZHOU Chun-shan CHEN Long-sheng |
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| Institution: | GAO Yi,ZHONG Shi-an*,ZHOU Chun-shan,CHEN Long-sheng |
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| Abstract: | A simple, rapid HPLC method was adapted to determine capsaicin in chilli(Capsicum annuum L.)with Burospher-100 C18 column(I.D.250mm×4.6mm,5μm) ,unsing MeOH-H2O(70:30,V/V)as mobile phase with an eluting rate of 0.6mL/min and detected at 280mm..There is a good linear relationship in the range of 1.01～121.2mg/L for capsaicin, and the correlative coefficient is 0.9994.When the mothod was applied to analyse chilli extract, and the relative standard deviation is 1.38%(n=6)and the average recovery is 99.88%. |
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| Keywords: | HPLC Capsicum aanuum capsaicin quantitative analyse |
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