苦橙叶精油化学成分及抗炎作用研究

为探究苦橙叶精油的抗炎作用。实验采用气相-质谱联用法(GC-MS)分析精油成分,并建立脂多糖(LPS)诱导RAW 264.7细胞炎症模型,用Griess法检测一氧化氮(NO)含量评价其体外抗炎作用,随后进一步通过巴豆油致小鼠耳肿胀模型和鸡蛋清致小鼠足肿胀模型评价其体内抗炎作用。结果表明苦橙叶精油成分以酯类、醇类物质为主;25μg/mL浓度能显著抑制RAW 264.7细胞NO的释放;中浓度苦橙叶精油能明显减轻小鼠耳肿胀程度;低、中、高浓度苦橙叶精油均对小鼠足肿胀模型有炎症缓解作用,并于肿胀前期呈浓度依赖性。以上实验证明苦橙叶精油在体外和体内具有一定抗炎作用。

Chemical composition and anti-inflammatory effect of essential oil from leaves of Citrus aurantium L.subspecies amara L.

This study is aimed to observe the anti-inflammatory effect of essential oil from leaves of Citrus aurantium L.subspecies amara L.Gas chromatography-mass spectrometry(GC-MS) results showed that linalyl acetate(54.72%) and linalool(22.98%) were the main constituents of this essential oil.The essential oil at concentrations of 25 μg/mL significantly inhibited the release of nitric oxide(NO) on the lipopolysaccharide(LPS)-induced inflammation model in RAW 264.7 macrophages measured by Griess method.Additionally,in animal experiments,essential oil from leaves of C.aurantium L.subspecies amara L.significantly relieved the croton oil induced mouse ear edema at the middle concentration.It also reduced the fresh egg white induced mouse paw edema and presented in a dose-dependent manner in the early stage of swelling.It indicated that essential oil from leaves of C.aurantium L.subspecies amara L.showed anti-inflammatory potential.