玫瑰色微球菌A-04的红色素鉴定及稳定性分析

从石油污染的土壤和水样中筛选出一株玫瑰色微球菌A-04 Micrococcus roseus,对其所产红色色素进行了分离,并初步鉴定了色素种类,基于对影响A-04色素稳定性的单因素分析基础之上,采用3因素3水平响应面分析法,进一步对影响色素稳定性的主要因素进行了优化分析。结果表明A-04所产红色色素为类胡萝卜素,对该菌株的色素稳定性的单因素条件分析,色素对环境条件的耐受性较好,在80℃、pH5. 0～8. 0等条件下依然能长时间保持鲜红而不褪色。经响应面优化分析表明:温度、pH和溶剂是影响该色素稳定性的主要因素,温度与溶剂的交互作用对色素稳定性的影响也较为明显。pH5. 0～8. 0之间时,在80℃范围内,温度越低,同时溶剂的极性越大,越有利于维持色素的稳定性。本研究结果为该色素的实际开发和应用奠定了基础。

Identification of the red pigment from Micrococcus roseus A-04 and the analysis of its stability

The purpose of this study was to identify a red pigment produced by Micrococcus roseus A-04 which was isolated from an oil-polluted soil and water and preserved by our laboratory.The factors influencing on the pigments’stability were analyzed by using the single factor experiment.Moreover,a response surface methodology with three factors and three levels was used in this study to investigate the key factors that influenced pigments’stability based on single factor analyses.The results showed that the red pigment from Micrococcus roseus A-04 belongs to carotenoids on the basis of spectroscopic analysis.The single factor experiment showed that the pigment exhibited high stability under a variety of conditions,and did not fade after a long time.This article explored the interactions between the factors and the pigments’stability.The results showed that temperature,pH and solvent are the key factors,and the interaction between temperature and solvent effects the stability of pigment obviously.Under 80℃and pH between 5 and 8,the lower temperature and stronger polarity is,the more stability the pigments will be.This work lays a foundation for the development and application of this pigment.