巴戟天中蒽醌类化合物及生物活性研究

采用硅胶柱层析结合制备液相从巴戟天(Morinda officinalis)中分离得到8个蒽醌类化合物。根据化合物的波谱数据并与文献对照进行了结构鉴定,分别为2-羟甲基-3-羟基蒽醌(2-hydroxymethyl-3-hydroxyanthraquinone,1)、3-羟基-2-羟甲基-1-甲氧基蒽醌(3-hydroxy-2-hydroxymethyl-1-methoxyanthraquinone,2)、2-羟基-1-甲氧基蒽醌(2-hydroxy-1-methoxyanthraquinone,3)、3-羟基-1,2-二甲氧基蒽醌(3-hydroxy-1,2-dimethoxyanthraquinone,4)、甲基异茜草素-1-甲醚(rubiadin-1-methyl ether,5)、1,3-二羟基-2-甲氧基蒽醌(1,3-dihydroxy-2-methoxyanthraquinone,6)、1,3-二羟基-2-乙氧甲基蒽醌(ibericin,7)、1,2-二羟基-3-甲基蒽醌(1,2-dihydroxy-3-methylanthraquinone,8)。其中蒽醌(2)为首次从该植物中分得。利用MTT法对分离出的蒽醌的体外抗癌活性进行筛选,结果显示蒽醌(3)、(5)和(7)对肝癌细胞SMMC-7721增殖有明显的抑制作用,当蒽醌的浓度为400μmol/L时,蒽醌(3)、(5)和(7)对肝癌细胞的抑制率分别为44. 63%、20. 52%、54. 89%。

Isolation,identification and bioactivity of anthraquinones in Morinda officinalis

Eight anthraquinones were isolated from the Chinese medicine:Morinda officinalis,using silica gel column chromatography and preparative HPLC.Those anthraquinones were identified as 2-hydroxymethyl-3-hydroxyanthraquinone(1),(3-hydroxy-2-hydroxymethyl-1-methoxyanthraquinone(2),2-hydroxy-1-methoxyanthraquinone(3),3-hydroxy-1,2-dimethoxyanthraquinone(4),rubiadin-1-methyl ether(5),1,3-dihydroxy-2-methoxyanthraquinone(6),ibericin(7)and 1,2-dihydroxy-3-methylanthraquinone(8)on the basis of spectral data and comparison with literatures.Anthraquinone(2)was isolated from this plant for the first time.The anticancer activities of those anthraquinones were screened by MTT assay.Results showed that three anthraquinones(3,5 and 7)had obvious inhibitory effects on the proliferation of hepatic carcinoma cell lines(SMMC-7721)in vitro.When the concentration of anthraquinone was 400 μmol/L,inhibitory rates of anthraquiones(3,5 and 7)against SMMC-7721 reached 44.63%,20.52% and 54.89%,respectively.