土壤真菌Curvularia affinis HS-FG-196抗肿瘤化学成分的再研究

为对土壤真菌Curvularia affinis HS-FG-196的次级代谢产物及其体外抗肿瘤活性进行进一步研究。实验采用大孔吸附树脂HP-20树脂柱、硅胶柱、凝胶LH-20柱及半制备高效液相色谱柱从Curvularia affinis HS-FG-196的发酵培养物中分离得到六个单体化合物(1～6)。利用~1H NMR、¹³C NMR、~1H-~1H COSY、HMQC、HMBC、IR、UV和MS等波谱分析方法对其进行了结构鉴定,分别是:pyrenocine S (1)、pyrenocine B (2)、pyrenocine E (3)、pyrenocine I (4)、pyrenochaetic acid B (5)和pyrenochaetic acid C (6),其中化合物1是新化合物。对所得单体化合物进行了体外抗肿瘤活性测试,结果显示化合物1、2、3对肿瘤细胞A549、HCT-116、ACHN、K562和HepG2表现出较强的活性。

Further study on the antitumor chemical constituents from soil fungus Curvularia affinis HS-FG-196

Six compounds(1-6) were obtained by using Diaion HP-20 column,silica gel column,Sephadex LH-20 column and semi-preparative HPLC during the further investigation on the chemical constituents of the soil fungus Curvularia affinis HS-FG-196.Their structures were determined on the basis of spectroscopic analysis,including ^1H NMR,^13C NMR,^1H-^1H COSY,HMQC and HMBC,as well as HR-TOF-MS and compared with the data reported in the literature.Pyrenocine S(1) was a new compound and the 5 known compounds were identified to be pyrenocine B(2),pyrenocine E(3),pyrenocine I(4),pyrenochaetic acid B(5) and pyrenochaetic acid C(6).The cytotoxicity of these compounds was assayed in vitro against the human tumor cells A549,HCT-116,ACHN,K562 and HepG2.As a result,compounds 1,2,3 showed strong cytotoxic activity and the remaining three compounds showed weak activity.