粘质沙雷氏菌S823次生代谢产物及其抗肿瘤活性研究

为从土壤中筛选得到能转化甾体化合物和产生具有抑制肿瘤生长的活性化合物菌株,并分离鉴定出活性化合物。利用胆固醇作唯一碳源筛选,通过形态观察、生理生化测试,以及16S rRNA基因序列同源性分析来确定筛选菌株。以Neuro-2a细胞(小鼠神经瘤细胞)作为供试细胞,通过活性跟踪,用柱层析法分离活性成分,经波谱方法鉴定产生的活性物质等方法。结果表明从土壤中筛选得到一株菌株具有抑制肿瘤生长的活性;此菌株与粘质沙雷氏菌属(Gen Bank No.序列号为KR817904. 1)相似性为99%,命名为粘质沙雷氏菌S823;活性化合物为灵菌红素,其对细胞周期分裂蛋白25B (CDC25B)的抑制率是67. 7±2. 9%,对含SH2结构域蛋白酪氨酸磷酸酶2(SHP2)抑制率为56. 7±0. 4%,对Neuro-2a细胞有抑制作用,其IC50是0. 05μM。灵菌红素是具有抗肿瘤活性的化合物。

Secondary metabolites of Serratia marcescans S823 and antitumor activities

In order to screen strains which can produce secondary metabolites of inhibition of tumor growth,and isolate and identify active compounds,cholesterol was used as the sole source of carbon to screen strains from soil.The active components of the strain S823 were separated by chromatography and structure of them was identified by spectrum analysis.The strain was determinated according to morphology,physiological and biochemical characteristics,and 16 s rRNA gene sequence homology analysis.The test results showed that the strain extract could inhibit Neuro-2 a cell(mice glioma cells)growth.The compound was isolated by column chromatography and its structure was identified as prodigiosin base on analysis of MS and 1H-NMR and 13 C NMR.The strain S823 was analyzed by morphological observation,physiological and biochemical characteristics,and 16S rRNA sequence analysis.GenBank No.is KR817904.1.The similarity of it is 99%to Serratia marcescens.The inhibition rate of prodigiosin against cell cycle protein 25 B(CDC25B)was 67.7±2.9%,the inhibition rate was 56.7±0.4%against the protein tyrosine phosphatase containing structure domain of SH2(SHP2).It has the inhibition effect on the Neuro-2 a cells,the value of IC 50 was 0.05μM.