美花石斛化学成分及其护肤活性研究

本文研究美花石斛(Dendrobium loddigesii)的化学成分及其护肤活性。采用色谱法从美花石斛粗提物中分离得到12个化合物,利用波谱学方法分别鉴定为拖鞋状石斛素(1)、2,4,7-三羟基-9,10-二氢菲(2)、3,6,9-三羟基-3,4-二氢蒽-1(2H)-酮(3)、3,5′-二甲氧基-3′,4,9′-三羟基-7′,9-环氧-8,8′-木酚素(4)、5-(4-羟基-3-甲氧基苯乙基)-2-(4-羟基-3-甲氧基苯基)苯并二氢呋喃-3,7-二醇(5)、5-(4′′-羟基-3′′-甲氧基苯乙基)-2-(4′-羟基-5′-甲氧基苯基)-7-甲氧基苯并二氢吡喃-3-醇(6)、丁香脂素(7)、异香草醛(8)、松柏醛(9)、2-甲氧基-5-羟甲基苯酚(10)、二氢松柏醇(11)、4-羟基-3-甲氧基苯乙醇(12)。其中化合物3～5、7～12为首次从美花石斛中分离得到;化合物1、2、6(100μg/mL)有一定的自由基清除活性(>80%);化合物2(10μg/mL)有一定促胶原蛋白分泌活性(>20%)。结果显示从美花石斛的茎中分离得到了12个化合物,3个表现出良好的护肤活性。

Chemical constituents and skin caring activities of Dendrobium loddigesii

To study the chemical constituents and explore the skin caring activity of Dendrobium loddigesii ,all compounds were isolated by chromatography with silica gel,Sephadex LH-20,MCI gel and Lichroprep RP-18 gel and identified by NMR and MS;Twelve compounds were isolated from D.loddigesii ,which were moscatin ( 1 ),2,4,7-trihydroxyl-9.10-dihydrophenanthrene ( 2 ),3,6,9-trihydroxy-3,4-dihydroanthracen-1(2H)-one ( 3 ),3,5′-dimethoxy-3′,4,9′-trihydroxy-7′,9-epoxy-8,8′-lignan ( 4),trigonopol B (5),crepidatuol B (6),syringaresinol (7),isovanillin (8),coniferyl aldehyde (9 ),2-methoxyphenol-5-hydroxymethyl ( 10),dihydroconiferyl alcohol (11 ),4-hydroxy-3- methoxyphenylethanol ( 12 ).And compound 3-5,7-12 were isolated from this plant for the first time.Compound 1,2,6 could scavenge almost 80% DPPH radical at 100 μg/mL,compound 2 could promote 25.6% secretion of collagen at 10 μg/mL.