基于高速逆流色谱的大极性海参多肽分离方法研究

本文通过对HEMWat溶剂体系中28种常用溶剂系统单相以及整体极性进行系统分析,探讨了无法用常规J型逆流色谱实现对大极性化合物实现有效分离的原因。根据分析结果建立了一种以分离物质理化性质为基础选择潜在改性剂,样品在溶剂系统上相中的溶解程度为判断指标进行筛选逆流色谱溶剂系统改性剂,进而得到适宜的溶剂系统对大极性海参多肽样品进行系统分段方法。经过两步分离,共得到8个随极性分布的海参多肽片段,为海参多肽最佳活性物质的追踪和筛选提供了技术支持。

Separation study of strong polar polypeptide from sea cucumber by high speed countercurrent chromatography

The reason why the strong polar compounds couldnt be well separated by conventional J type high speed countercurrent chromatography has been studied by analyzing the total and single phase’s polarity distribution rules of commonly used 28 kinds HEMWat solvent systems in this paper.According to the results,an effective sample pre-treatment method was established to segment the Sea Cucumber polypeptides.The method is based on the polypeptidesphysical and chemical properties to find a suitable modifier improving the distribution behavior of the samples in mobile phase and stationary phase.After two-step separation,eight sea cucumber polypeptide fractions with different polarity were obtained,which provided technical support for the tracking and screening of the best active substance of sea cucumber polypeptide.