复方安神精油吸入给药的镇静催眠作用及化学成分的GC-MS分析

为研究复方安神精油吸入给药的镇静催眠作用,本实验采用动物自主活动测试和戊巴比妥钠诱导的睡眠潜伏期与睡眠时间实验:GC-MS分析复方安神精油的挥发性化学成分与入脑的主要化学成分;基于网络药理学预测和筛选出治疗失眠症相关靶蛋白与活性成分,旨在为复方安神精油治疗与改善失眠症提供理论依据。研究发现,复方安神精油具有镇静催眠作用,可显著减少动物自主活动,缩短睡眠潜伏期,延长睡眠时间;复方安神精油主要化学成分与入脑主要化学成分中含有多种具有神经中枢镇静催眠作用以及抗焦虑、抗抑郁作用的化学成分;基于网络药理学预测和筛选出25个治疗失眠症相关活性成分作用于39个治疗失眠症相关作用靶点,体现复方安神精油多成分、多靶点发挥镇静催眠作用的特点。

Sedative and hypnotic effects of inhalation of compound anshen essential oil and GC-MS analysis of chemical constituents

In order to study the sedative and hypnotic effects of inhalation administration of compound anshen essential oil,this experiment used animal autonomic activity test and pentobarbital sodium-induced sleep latency and sleep time experiments.GC-MS analysis of the volatile chemical constituents of the compound anshen essential oil and the main chemical components into the brain,based on network pharmacology prediction and screening for the treatment of insomnia-related target proteins and active ingredients.It aims to provide a theoretical basis for the treatment of compound anshen oil and improve insomnia.The study found that the compound anshen essential oil has sedative and hypnotic effect,which can significantly reduce animal autonomic activity,shorten sleep latency and prolong sleep time.The main chemical components of compound anshen essential oil and the main chemical components into the brain contain many kinds of chemical components which have the sedative and hypnotic effect on the nerve center,anti-anxiety effect and anti-depression effect.Based on network pharmacology prediction and screening,25 insomnia-related active ingredients acted on 39 therapeutic insomnia-related targets,reflecting the characteristics of multiple components and multiple targets of compound anshen essential oils to exert sedative and hypnotic effects.