| 动物源活性蛋白多肽的分离纯化方法研究进展 |
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| 引用本文： | 付文鹏,杨永寿,肖培云.动物源活性蛋白多肽的分离纯化方法研究进展[J].天然产物研究与开发,2019(5):916-921. |
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| 作者姓名： | 付文鹏 杨永寿 肖培云 |
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| 作者单位： | 大理大学药学与化学学院;云南省昆虫生物医药研发重点实验室 |
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| 基金项目： | 国家自然科学基金(81360634;81560634);云南省科技厅应用基础研究重点项目(2015FA025) |
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| 摘 要： | 动物类中药的有效成分以蛋白多肽为主,因此活性蛋白多肽具有重要的医疗保健价值。文章分析了沉淀法、色谱法、膜分离法以及电泳法的基本原理和主要适用范围,综述了这些方法在动物源活性蛋白多肽的分离纯化中的应用,为动物源蛋白多肽的分离纯化与进一步研究提供参考,以期开发出高效、经济和环保的蛋白多肽分离纯化新技术。
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| 关 键 词： | 蛋白质 多肽 分离 纯化 研究进展 |
Research progress on isolation and purification methods of animal-derived active proteins and polypeptides |
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| FU Wen-peng,YANG Yong-shou,XIAO Pei-yun.Research progress on isolation and purification methods of animal-derived active proteins and polypeptides[J].Natural Product Research and Development,2019(5):916-921. |
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| Authors: | FU Wen-peng YANG Yong-shou XIAO Pei-yun |
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| Institution: | (School of Pharmacy and Chemistry,Dali University;Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D,Dali 671000,China) |
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| Abstract: | The active ingredients of animal traditional Chinese medicines are mainly proteins and polypeptides,so active proteins and polypeptides have important medical and health value.In this paper,the basic principles and main application scopes of precipitation method,chromatography,membrane separation method and electrophoresis method are analyzed.The application of these methods in the separation and purification of animal-derived active proteins and polypeptides is reviewed.This provides a reference for the separation,purification and further research of animal-derived proteins and polypeptides,in order to develop a new technology for the separation and purification of proteins and polypeptides with high efficiency,economy and environmental protection. |
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| Keywords: | proteins polypeptides separation purification research progress |
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