| 栀子组织和细胞培养生产天然食用色素的研究——Ⅰ.愈伤组织培养的研究 |
| |
| 引用本文： | 钟青萍,杨宁生,刘敏.栀子组织和细胞培养生产天然食用色素的研究——Ⅰ.愈伤组织培养的研究[J].天然产物研究与开发,1994(4). |
| |
| 作者姓名： | 钟青萍 杨宁生 刘敏 |
| |
| 作者单位： | 江西中德联合研究院，江西中德联合研究院，江西中德联合研究院 南昌 330047，南昌 330047，南昌 330047 |
| |
| 摘 要： | 在诱导出愈伤组织的基础上,对各种不同的培养条件进行分析研究,考察它们对愈伤组织生长和栀子黄色素产生的影响,筛选出适宜的生长培养基:B_5+IBA 1mg/l+KT0.23mg/l、MG-5+IBA 1mg/l+KT 0.23mg/l、B_6+IAA 1.5mg/l和生产培养基:M-9+IAA 1mg/l、M-9+IAA 1.5mg/l.并且,获得了几个色素含量较高的愈伤组织系。另外,还研究了含色素和不含色素的愈伤组织在培养过程中的过氧化物同工酶的差异。
|
| 关 键 词： | 栀子 愈伤组织培养 色素产生 过氧化物同工酶 |
STUDY ON TISSUE CULTURE OF GARDENIA JASMINOIDES ELLIS TO PRODUCE FOOD PIGMENT |
| |
| Zhong Qingping,Yang Ningsheng,Liu Min.STUDY ON TISSUE CULTURE OF GARDENIA JASMINOIDES ELLIS TO PRODUCE FOOD PIGMENT[J].Natural Product Research and Development,1994(4). |
| |
| Authors: | Zhong Qingping Yang Ningsheng Liu Min |
| |
| Abstract: | In this paper different culture factors for callus induction growth and pigment formation of Gardenia jasminoides Ellis,were studied In-view of the effects of the factors,two-step culture system war formed.The best growth media were B5+IBAlmg/l + KT0.23mg/l,MG-5+IBAlmg/l + KT 0.23mg/l and B54-IAAl.5mg/l,The suitable production media were M-9+IAAlmg/l and M-9 + IAA1.5mg/l in which the calli could produce pigment.Mean time,several callus lines with high pigment content were selected The difference of the isozyme patterns of peroxidase between the yellow callus and the white callus was also studied. |
| |
| Keywords: | Gardenia jasminoides Kllis Tissue culture pigment formation peroxidase |
| 本文献已被 CNKI 等数据库收录！ |
