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| 紫杉烷类化合物的抗氧化应激作用(英文) | |
| 引用本文： | 段瑞生,董玫,王维平,刘娜,贾丽景,甄军丽,黄晓.紫杉烷类化合物的抗氧化应激作用(英文)[J].天然产物研究与开发,2010,22(2). |
| 作者姓名： | 段瑞生 董玫 王维平 刘娜 贾丽景 甄军丽 黄晓 |
| 作者单位： | 1. 河北医科大学第二附属医院神经内科,石家庄,050000 2. 河北医科大学法医教研室,石家庄,050017 3. 石家庄医学高等专科学校外科教研室,石家庄,050081 4. 保定市第一中心医院,保定,071000 |
| 基金项目： | supported from FRND of China (No: 2003AA2Z3527); Scientific Research Foundation for the Returned Overseas Chinese Scholars from Hebei Province and State Education Ministry of P.R.China to QWS |
| 摘 要： | 为探讨紫杉烷类化合物的抗氧化应激作用,选择适当浓度双氧水处理神经母细胞瘤细胞(SK-N-SH)建立氧化应激细胞模型,用自红豆杉科的植物(Taxus cuspidate)中分离纯化的四种紫杉烷类化合物Taxine B、7-Deacetyl-taxine B、5-Cinnamoyloxy-taxin B、7,10-Diacetyl-2′-deoxyl-taxine A干预过氧化应激模型细胞,用四甲基偶氮唑盐(3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide,MTT)微量酶反应比色法筛选具有神经保护活性的化合物,Hoechst荧光染色观察细胞核形态改变,DCFH-DA检测细胞内活性氧含量.发现氧化应激模型细胞活力明显减低,应用7-Deacetyl-taxine B、5-Cinnamoyloxy-taxin B后细胞活力显著提高,细胞核形态改变明显减轻,细胞内活性氧含量明显减少.结果表明7-Deacetyl-taxine B、5-Cinnamoyloxy-taxin B可以减少细胞内氧自由基含量,减轻细胞核损伤,提高细胞活力. |
| 关 键 词： | 紫杉烷化合物 神经保护作用 氧化应激 过氧化氢 氧自由基 羟基 |
Anti-oxidative Effects of Taxanes |
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| DUAN Rui-sheng,DONG Mei,WANG Wei-ping,LIU Na,JIA Li-jing,ZHEN Jun-li,HUANG Xiao.Anti-oxidative Effects of Taxanes[J].Natural Product Research and Development,2010,22(2). | |
| Authors: | DUAN Rui-sheng DONG Mei WANG Wei-ping LIU Na JIA Li-jing ZHEN Jun-li HUANG Xiao |
| Institution: | DUAN Rui-sheng1,DONG Mei2,WANG Wei-ping1,LIU Na3,JIA Li-jing1,ZHEN Jun-li1,HUANG Xiao41The Second Hospital of Hebei Medical University,Neurology Department,Shijiazhuang City 050000,2Forensic Department of Hebei Medical University,3Surgical Department of Shijiazhuang Medical College,Shijiazhuang City 050081,4Baoding City No.1 Central Hospital,Baoding City 071000 |
| Abstract: | Object:to study the anti-oxidative effects of taxanes,we used suitable concentration of hydrogen peroxide to establish the oxidative sk-n-sh cell model and added four taxanes compounds(Taxine B,7-Deacetyl-taxine B,5-Cinnamoyloxy-taxin B and 7,10-Diaectyl-2′-deoxyl-taxine A)to intervene the oxidative stress 2 hrs before hydrogen peroxide application.Their neuroprotective effects were evaluated using 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliummmide(MIT)assay,their effects of alleviating the nuclear morphological changes were detected after Hecchst 33258 staining and the activity ofdecreasing intracellular reactive oxygen species(Ros)was surveyed with flow cytometry.We discovered that 7-Deacetyl-taxine B and 5-Cinnamoyloxy-taxin B significantly improved cell viability,decreased obviously the number of cells with pycnosised neuclei and reduced intracellular ROS remarkably comparing with oxidative stress model.So we got the conclusion that 7-Deacetyl-taxine B,5-Cinnamoyloxy-taxin B can reduce intracellular ROS,protect cells neucleis and improve viability of oxidative model cells. |
| Keywords: | taxane neuroprotective effect oxidative stress hydrogen peroxide reactive oxygen species hydroxyl group |
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