稀有人参皂苷IH901酶法转化与制备研究

本研究利用酶制剂蜗牛酶,酶法转化三七二醇组皂苷制备稀有人参皂苷IH901,正交实验优化酶解条件,建立酶法转化工艺.结果表明:超声法提取三七总皂苷正交实验优化条件为用75%乙醇溶液,15倍溶剂用量,超声波提取210 min作为最佳条件,三七总皂苷得率为12.21%;酶法转化二醇组人参皂苷制备稀有人参皂苷IH901,正交实验优化的条件为物料比为6/1、反应时间9 h、反应温度为45℃、pH值为3.0,酶解得率为54.24%;经硅胶柱分离获得IH901单体化合物,HPLC测定纯度达98%.酶法转化制备皂苷IH901的工艺方法简便,切实可行,可为中试生产提供参考.

Study on Enzymatic Transformation and Preparation of Rare Ginsenoside JH901

Rare ginsenoside IH901 was preparated through enzymatic transformation from protopanaxadiol ginsenoside of Panax notoginseng by snailase,hydrolysis conditions were optimized by the orthogonal test,the enzymatic conversion process was established.The results showed that orthogonal test for optimization conditions of Ultrasonic extraction of Panax notoginoside(PNS)was solvent for 75% ethanol solution,IS times the amount of solvent,210 min ultrasonic extraction as the best optimization of conditions,PNS was 12.21 percent rate;the affecting factors of enzymatic transformation were studied and optimized,and the best optimal conditions were obtained as following:6/1 for material ratio,9 h for reaction time,45 ℃ as reaction temperature and 3.0 as pH value,the optimum rate of crude hydrolysate was 54.24%.The ginsenoside IH901 was purified by silica gel column chromatogram.its purity was about 98% analyzed by HPLC.Enzymatic conversion process of IH901 preparation is simple,practical,can provide a reference in the trial production.