| 雷公藤植物组织培养物的生长及其中二萜内酯积累动态的研究 |
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| 引用本文： | 尹作鸿,朱蔚华.雷公藤植物组织培养物的生长及其中二萜内酯积累动态的研究[J].天然产物研究与开发,1991(2). |
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| 作者姓名： | 尹作鸿 朱蔚华 |
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| 作者单位： | 中国医学科学院药物研究所，中国医学科学院药物研究所 北京 100050，北京 100050 |
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| 摘 要： | 培养的雷公藤植物组织经不断筛选获得优良无性系D_(121(y))。经测定,该无性系在培养后的第七周干重、鲜重、二萜内酯含量、二萜内酯的产量达到最大值;在第五周,培养细胞生长最快;在第七周,干燥率产生一个峰值。因此,雷公藤组织培养的最佳转接时期为培养的第五周,以收获二萜内酯为目的的最佳收获期应在培养后的第七周。
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| 关 键 词： | 雷公藤 组织培养 生长 二萜内酯 积累 |
STUDIES OF THE TIME COURSES OF GROWTH AND DITERPENELACTONE ACCUMULATION IN TRIPTERYGIUM WILFORDII HOOK F |
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| Yin Zhuohong,Chu Weihua.STUDIES OF THE TIME COURSES OF GROWTH AND DITERPENELACTONE ACCUMULATION IN TRIPTERYGIUM WILFORDII HOOK F[J].Natural Product Research and Development,1991(2). |
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| Authors: | Yin Zhuohong Chu Weihua |
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| Abstract: | Cell line D_(121(y)) of high growth and high-produetion diterpene-lactone was selected. It was sure that calluses produced diterpene-Iactone identified by Kedde reaction and TLC. In the seventh week, the dry weight, fresh weight, content and production of diterpene-Iactone in the calluses reached the highest value; In the fifth week, calluses grew fastest; In the seventh week, dry rate of calluses reached a highest value. The harvest time should be in the seventh week if diterpene-Iactone should be obtained. |
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| Keywords: | Tripterygium wilfordii growth accumulation diterpenelactone tissue culture |
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