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| 大孔吸附树脂分离富集苜蓿皂甙的研究 | |
| 引用本文： | 古燕翔,张国良,魏芸,王松柏,李桂荣,胡跃高.大孔吸附树脂分离富集苜蓿皂甙的研究[J].天然产物研究与开发,2007,19(3):504-507. |
| 作者姓名： | 古燕翔 张国良 魏芸 王松柏 李桂荣 胡跃高 |
| 作者单位： | 1. 北京市延庆县种植业服务中心,北京,102100;中国农业大学农学与生物技术学院,北京,100094 2. 中国农业科学院农业环境与可持续发展研究所,北京,100081 3. 北京化工大学理学院,北京,100029 4. 中国农业大学农学与生物技术学院,北京,100094 |
| 摘 要： | 本项工作以对苜蓿皂甙的吸附量和解吸率为指标筛选大孔吸附树脂。研究结果表明,X-5吸附树脂具有较好的吸附性能和解吸效果。研究应用正交试验方法进一步对大孔吸附树脂分离纯化苜蓿皂甙的工艺条件进行试验分析,确定苜蓿皂甙分离富集的最佳操作条件为:上样浓度8mg/mL,色谱柱的径高比1∶7,药材-树脂比例1∶3;吸附完全后,先以水洗脱,除去杂质,再以50%乙醇洗脱,可以得到纯度较好的苜蓿皂甙。 |
| 关 键 词： | 苜蓿皂甙 分离富集 X-5大孔吸附树脂 |
| 文章编号： | 1001-6880（2007）02-0504-04 |
| 收稿时间： | 2006-01-11 |
| 修稿时间： | 2006-01-112006-02-22 |
Study on Isolation and Purification of Alfalfa Saponins by Macroporous Resin |
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| GU Yan-xiang,ZHANG Guo-liang,WEI Yun,WANG Song-bai,LI Gui-rong,HU Yue-gao.Study on Isolation and Purification of Alfalfa Saponins by Macroporous Resin[J].Natural Product Research and Development,2007,19(3):504-507. | |
| Authors: | GU Yan-xiang ZHANG Guo-liang WEI Yun WANG Song-bai LI Gui-rong HU Yue-gao |
| Institution: | 1.Planting Industry Service Centre of Yanqing County of Beijing,Beijing 102100,China; 2.Institute of Agriculture and Biology Technology of Chinese Agricultural University, Beljing 1000942, China; 3.Institute of Environment and Sustainable Development in Agricultural of Chinese Academy of Agricultural Sciences, Beijing 100081, China; 4.Bering University of Chemical Technology, Bering 100029, China |
| Abstract: | To isolate and purify alfalfa saponin static absorption test and desorption test were carried out to select the macroporous resin.Optimal purification process of alfalfa saponins was determined by single factor test,orthogonal experimental designs and variance analysis.The macroporous resin X-5 not only possesses the strongest absorption ability to all alfalfa saponins among the 7 resins studied,but also has an easy desorption property.The optimum absorption conditions were as follows:concentration of loading sample is 8 mg/mL,the ratio of the chromatography column diameter to height is 1:7,and the weight ratio of raw material to resin is 1:3. |
| Keywords: | alfalfa saponins technical condition X-5 macroporous resin |
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