大孔吸附树脂纯化海红果黄酮工艺的研究

通过采用大孔吸附树脂对海红果黄酮粗提液的静态吸附和解吸试验,从10种大孔吸附树脂中筛选出海红果黄酮纯化的最优树脂,考察了该树脂对诲红果黄酮的静态、动态吸附与解吸性能并对吸附与洗脱的最佳条件进行了研究.结果表明:NKA-9树脂对海红果黄酮有很好的吸附和解吸性能,其最优的动态吸附工艺条件为:上样液pH值为4.0,浓度5.15 mg/mL,上样量为4 BV,流速控制在2 BV/h.最优的解吸工艺条件为:洗脱剂为80％乙醇溶液,洗脱液用量为3 BV,洗脱流速控制在1 BV/h.在此优化条件下,海红果黄酮的吸附率、解析率、收率、纯度的平均值分别达到为(79.39±0.13)％,(84.14±0.11)％,(68.20±0.15)％和(28.81 ±0.06)％ (n=5).

Purification Technology of Malus micromalus Makino Flavonoids by Macroporous Absorbent Resins

The suitable resin was selected by static and dynamic adsorption from 10 kinds of macroporous absorbent resins to Malus micromalus Makino flavonoids.The purification technology of the crude extracts by macroporous adsorbing resins were decided through the experiments.The results showed that NKA-9 resin was selected because it gave the most effective of separation.The optimum process for the purification was as follows:the concentration of sample solution was 5.15 mg/mL,pH was 4.0,the loading weight was 4 BV and the adsorption flow rate was 2 BV/h;then eluted with 80% ethanol for 3 BV with elution flow rate of 1 BV/h.In these optimum conditions,average adsorption rate,desorption rate,yield and purity of the flavonoids was reached to(79.39±0.13)%,(84.14±0.11)%,(68.20±0.15)% and(28.81±0.06)%(n=5),respectively.