| 茶碱与胃蛋白酶相互作用的光谱性质研究 |
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| 引用本文： | 谢光,刘芯韵,郑芸,裘晓丹,刁家志,刘克武.茶碱与胃蛋白酶相互作用的光谱性质研究[J].天然产物研究与开发,2006,18(4):558-561. |
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| 作者姓名： | 谢光 刘芯韵 郑芸 裘晓丹 刁家志 刘克武 |
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| 作者单位： | 四川大学生命科学学院,生物资源与生态环境教育部重点实验室,成都,610064 |
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| 摘 要： | 采用紫外光谱法和荧光光谱法研究了茶碱与胃蛋白酶的结合作用。观测到茶碱使胃蛋白酶的紫外吸收峰增强,特征荧光峰淬灭。Stern-Volmer淬灭曲线显示,茶碱对胃蛋白酶的荧光淬灭很可能是一个单一的静态淬灭过程。
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| 关 键 词： | 茶碱 胃蛋白酶 紫外光谱 荧光光谱 |
| 文章编号： | 1001-6880（2006）04-0558-04 |
| 收稿时间： | 2005-10-31 |
| 修稿时间： | 2005-11-28 |
Spectroscopy Study on the Interaction between Theophylline and Pepsin |
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| XIE Guang,LIU Xin-yun,ZHENG Yun,QIU Xiao-dan,DIAO Jia-zhi,LIU Ke-wu.Spectroscopy Study on the Interaction between Theophylline and Pepsin[J].Natural Product Research and Development,2006,18(4):558-561. |
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| Authors: | XIE Guang LIU Xin-yun ZHENG Yun QIU Xiao-dan DIAO Jia-zhi LIU Ke-wu |
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| Institution: | Key Laboratory of Bio-resources and Eco-environment, College of Life Sciences, Sichuan University, Chengdu 610064, China |
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| Abstract: | The binding interaction between theophylline and pepsin was studied by ultraviolet absorption and fluorescence spectra.Theophylline treatment led to the increase of UV absorption and the quenching of intrinsic fluorescence of pepsin.The Stern-Volmer curve showed that the quenching of theophylline to pepsin was probably a single static quenching process. |
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| Keywords: | theophylline pepsin ultraviolet spectra fluorescence spectra |
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