| 肉苁蓉细胞悬浮培养的代谢动力学研究 |
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| 引用本文： | 李春,李娟,沈慧慧,易丽娟,刘慧英.肉苁蓉细胞悬浮培养的代谢动力学研究[J].天然产物研究与开发,2007,19(2):193-197. |
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| 作者姓名： | 李春 李娟 沈慧慧 易丽娟 刘慧英 |
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| 作者单位： | 1. 石河子大学生物化工实验室,石河子,832003;北京理工大学生命科学与技术学院,北京,100081
2. 石河子大学生物化工实验室,石河子,832003 |
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| 基金项目： | 石河子大学工科振兴计划专项基金 |
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| 摘 要： | 通过研究肉苁蓉细胞悬浮培养过程中碳源、氮源、磷源的消耗,pH、电导率的变化,以及细胞的生长、胞内外蛋白质含量和次生代谢产物苯乙醇苷、总黄酮和多糖合成的情况,掌握了细胞生长、营养消耗与次生代谢产物积累的基本规律,为建立结构化动力学模型奠定了基础。
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| 关 键 词： | 悬浮培养 代谢动力学 苯乙醇苷 肉苁蓉 |
| 文章编号： | 1001-6880（2007）02-0193-05 |
| 收稿时间： | 2006-01-23 |
| 修稿时间： | 2006-02-27 |
Metabolic Dynamic of Suspension Culture of Cistanche deserticola |
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| LI Chun,LI Juan,SHEN Hui-hui,YI Li-juan,LIU Hui-ying.Metabolic Dynamic of Suspension Culture of Cistanche deserticola[J].Natural Product Research and Development,2007,19(2):193-197. |
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| Authors: | LI Chun LI Juan SHEN Hui-hui YI Li-juan LIU Hui-ying |
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| Institution: | 1. Laboratory of Biochemical Engineering, Shihezi University, Shihezi 832003, China; 2.School of Life Science and Technology,Beijing Institute of Technology, Beijing 100081, China |
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| Abstract: | In the suspension culture of Cistanche deserticola,the consumption of nutrition substrate including carbon,nitrogen and phosphate source were investigated.The changes of pH and conductance rate,cell growth,soluble proteins and secondary products including phenylethanoid glycosides,total flavones and polysaccharides were analyzed.The regulations among growth of cell,the consumption of nutrition and the accumulation of secondary metabolites were grasped.All these could be a basis for establishing the structural dynamic model and optimizing the culture conditions. |
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| Keywords: | suspension culture metabolic dynamic phenylethanoid glycosides Cistanche deserticola |
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