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迎春花中黄酮类化合物含量的研究
引用本文:郑敏燕,魏永生.迎春花中黄酮类化合物含量的研究[J].天然产物研究与开发,2003,15(6):494-497.
作者姓名:郑敏燕  魏永生
作者单位:咸阳师范学院化学系,咸阳,712000
摘    要:以75%乙醇为溶剂,索氏提取器提取,采用比色法和HPLC法研究了迎春花中黄酮类化合物的含量。以芦丁为对照物,比色法测定出其花、叶、茎中总黄酮的含量分别为13.7%、16.2%、6.77%.RSD分别为2.63%、2.57%、2.96%;以槲皮素、山奈素、异鼠李素为对照物,HPLC法测定出其花、叶、茎中的黄酮总量,以甙元计分别为4.205%、3.371%、1.703%,RSD分别为1.76%、1.58%、1.98%。

关 键 词:迎春花  黄酮  比色法  HPLC法

STUDIES ON THE CONTENT OF FLAVONOIDS IN JASMINUM NUDIFLORUM LINDL.
ZHENG Min-yan,WEI Yong-sheng.STUDIES ON THE CONTENT OF FLAVONOIDS IN JASMINUM NUDIFLORUM LINDL.[J].Natural Product Research and Development,2003,15(6):494-497.
Authors:ZHENG Min-yan  WEI Yong-sheng
Abstract:The flavonoids in Jasminum nudiflorum Lindl. extracted by Soxhlet extractor with 75% ethanol was studied by colorimetry and HPLC. Determined by colorimetry with rutin as standard sample, the content of total flavonoids in its flowers, leaves and stems were 13.7%,16.2% and 6.77%,RSD were 2.63%, 2.57% and 2.96% respectively, while determined by HPLC with quercetin, kaempferol, isorhamnetin as standard samples, the content of total flavonoids in its flowers, leaves and stems counted by aglycone were 4.205%, 3.371%, 1.703%, RSD were 1.76%, 1.58% and 1.98% respectively.
Keywords:Jasminum nudiflorum Lindl    flavonoids  HPLC  colorimetry
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