化学模式识别结合HPLC指纹图谱的僵蚕质量评价研究

建立HPLC指纹图谱结合化学模式识别技术对僵蚕药材进行质量评价。采用Insertsil ODS-3(4.6 mm×250 mm,5μm)色谱柱,以乙腈-0.1%磷酸水溶液为流动相梯度洗脱,检测波长365 nm,流速0.8 mL/min,进样量10μL,柱温30℃。采用相似度评价、聚类分析、主成分分析等化学模式识别方法,对不同来源的20批僵蚕药材进行质量分析和评价。建立的指纹图谱标定了9个共有峰,指认了5个峰,1号峰为芦丁,2号峰为金丝桃苷,4号峰为紫云英苷,6号峰为槲皮素,7号峰为山奈酚,并将9个共有峰峰面积与其性状、总灰分、酸不溶性灰分、浸出物及白僵菌素含量进行相关性分析。结果表明本研究建立的僵蚕HPLC指纹图谱结合化学模式识别的方法可靠、易行,方法重复性好、专属性强,与药材性状、总灰分、浸出物及白僵菌素含量等质控指标均有一定的相关性,为后续僵蚕药材的质量控制提供依据和参考。

Study on quality evaluation of Bombyx Batryticatus by chemical pattern recognition combined with HPLC fingerprint

HPLC fingerprint and chemical pattern recognition technology were used to evaluate the quality of Bombyx Batryticatus.Insertsil ODS-3 column(4.6 mm×250 mm, 5 μm) was used for gradient elution with acetonitrile-0.1% phosphoric acid solution as mobile phase.The detection wavelength was 365 nm, and flow rate was 0.8 mL/min.The injection volume was 10 μL,and column temperature was 30 ℃.Similarity evaluation, Cluster analysis(CA),and principal component analysis(PCA),etc, were used to analyze and evaluate the quality of 20 batches of samples from different areas.Nine common peaks were confirmed in the fingerprint.Five chromatographic peaks were identified, rutin, hyperin, rhodoside, quercetin and kaempferol.In addition, the correlation analysis were uesd between nine common peak areas and their appearance characters, total ash, acid-insoluble ash, extract and Beauverin content.The results showed that the HPLC fingerprint of Bombyx Batryticatus established in this study combined with chemical pattern recognition is simple and convenient, reliable, repeatable and specific, and has a certain correlation with the quality control indexes such as the character of medicinal materials, total ash, extract and Beauverin, which provides the basis and reference for the follow-up quality control of Bombyx Batryticatus.