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| 银耳多糖单糖组成分析的三种色谱方法比较 | |
| 引用本文： | 韩威,姜瑞芝,陈英红,高阳,高其品.银耳多糖单糖组成分析的三种色谱方法比较[J].天然产物研究与开发,2012,24(3):359-361,302. |
| 作者姓名： | 韩威 姜瑞芝 陈英红 高阳 高其品 |
| 作者单位： | 1. 延边大学,延吉,133002 2. 吉林省中医药科学院,长春,130012 3. 长春中医药大学,长春,130117 |
| 基金项目： | 十一五国家科技重大专项,国家自然科学基金项目 |
| 摘 要： | 为选择一种准确快捷的方法测定银耳多糖的单糖组成,对薄层色谱法(TLC)、气相色谱法(GC)、高效液相色谱法(HPLC)三种色谱方法进行比较。结果表明,前两种方法的测定结果均不理想,而HPLC法,操作简便,灵敏度高,分离效果好,信息完整。测定结果为由葡萄糖、甘露糖、葡萄糖醛酸、木糖、岩藻糖组成,其摩尔比为0.24∶1.00∶0.06∶0.29∶0.25。HPLC法对酸性杂多糖组成糖分析是一种比较理想的选择。 |
| 关 键 词： | 银耳多糖 单糖组成 TCL GC HPLC |
Comparison of Three Chromatographic Methods for Monosaccharide Composition Analysis of Tremella Polysaccharide |
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| HAN Wei , JIANG Rui-zhi , CHEN Ying-hong , GAO Yang , GAO Qi-pin.Comparison of Three Chromatographic Methods for Monosaccharide Composition Analysis of Tremella Polysaccharide[J].Natural Product Research and Development,2012,24(3):359-361,302. | |
| Authors: | HAN Wei JIANG Rui-zhi CHEN Ying-hong GAO Yang GAO Qi-pin |
| Institution: | 1Yanbian University,Yanji 133002,China;2Academy of Traditional Chinese Medicine and Material Medical of Jilin Province,Changchun 130012,China;3Changchun University of Chinese Medicine,Changchun 130117,China |
| Abstract: | To find an accurate and fast method to determine the monosaccharide composition of Tremella polysaccharide,thin layer chromatography(TLC),gas chromatography(GC) and high performance liquid chromatography(HPLC) were compared.The results of TLC and GC were not ideal.However,HPLC was a simple method with high sensitivity,good resolution and integrity information.The result of HPLC analysis showed that the monosaccharide compositions were Glc,Man,GlcA,Xyl and Fuc,with the mole percentage of 0.24∶ 1.00∶ 0.06∶ 0.29∶ 0.25.Consequently,HPLC is the most suitable method for monosaccharide composition analysis. |
| Keywords: | Tremella polysaccharide monosaccharide compositions TLC GC HPLC |
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