| 石油降解菌X-1所产表面活性剂的研究 |
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| 引用本文： | 张娜,隆小华,刘兆普,牛丹丹,姚瑶,王博.石油降解菌X-1所产表面活性剂的研究[J].天然产物研究与开发,2012,24(3):389-392. |
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| 作者姓名： | 张娜 隆小华 刘兆普 牛丹丹 姚瑶 王博 |
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| 作者单位： | 南京农业大学资源与环境科学学院江苏省海洋生物学重点实验室,南京,210095 |
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| 基金项目： | 公益性行业(农业)科研专项经费项目 |
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| 摘 要： | 对石油污染土壤中筛选到的能产生生物表面活性剂的石油降解菌X-1(芽孢杆菌),进行表面活性剂的提取和鉴定,并对其产剂条件进行了优化。结果表明:菌株X-1产生的生物表面活性剂为浅黄色粉末状物质。通过硅胶板薄层层析和红外光谱分析,判定表面活性剂为脂肽、脂蛋白类物质。菌株X-1产生表面活性剂的最佳条件为:温度32℃,pH 7.0,盐度2 g/L NaCl,最佳碳源为淀粉,最佳氮源为蛋白胨。
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| 关 键 词： | 石油降解菌 生物表面活性剂 薄层层析 红外光谱 脂肽类 优化 |
Biosurfactant Produced by Oil-degrading Bacteria X-1 |
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| ZHANG Na
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LONG Xiao-hua
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LIU Zhao-pu
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NIU Dan-dan
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YAO Yao
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WANG Bo.Biosurfactant Produced by Oil-degrading Bacteria X-1[J].Natural Product Research and Development,2012,24(3):389-392. |
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| Authors: | ZHANG Na LONG Xiao-hua LIU Zhao-pu NIU Dan-dan YAO Yao WANG Bo |
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| Institution: | Key Laboratory of Marine Biology of Jiangsu Province,College of Resources and Environmental Science,Nanjing Agricultural University,Nanjing 210095,China |
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| Abstract: | The oil-degrading bacteria X-1(Bacillus sp.) with capability of generating biosurfactant was isolated from petroleum-contaminated soil.The biosurfactant produced by the bacteria X-1 was extracted and identified.And the optimal conditions on biosurfactant production were studied.The results showed that the biosurfactant from strain X-1 was light yellow powdery material.By silica gel TLC and FT-IR analysis,the biosurfactant was determined as lipopeptid and lipoproteins.The optimal conditions for biosurfactant production were 32 ℃,pH 7.0 and 2 g/L NaCl.And the best carbon source and nitrogen source were starch and peptone,respectively. |
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| Keywords: | oil-degrading bacteria biosurfactant TLC FT-IR ipopeptid optimization |
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